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Skip to contentProduct Name: TB‑500 (Thymosin Beta‑4)
Category: Synthetic peptide for research use only
Intended Use: In vitro research, cell migration and tissue repair investigations
Source: Recombinant or synthetic peptide preparation
Appearance: Lyophilized (freeze‑dried) white to off‑white powder
Molecular Formula: C₆₅₈H₁₀₅₁N₁₆₇O₂₀₅S₅ (approximate — dependent on sequence variant)
Molecular Weight: ~4,960 Da (approximate)
Amino Acids: 43‑residue peptide sequence (human thymosin beta‑4 fragment)
CAS Number (if assigned): Not universally standardized, may vary by supplier
Common Synonyms: TB500, Thymosin β4 fragment
Use Restriction: For in vitro research only — Not approved for therapeutic or human use
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Sequence & Structure
Synthetic peptide representing the active fragment of the natural Thymosin Beta‑4 protein
Designed to promote cellular migration and repair pathways in research models
Purity Standards
Typical purity: ➤ ≥98 % by HPLC analysis (often reported as high as ≥99 %)
Confirmed by analytical HPLC and/or mass spectrometry
Low levels of detectable impurities
Assay / Activity
Verified by HPLC peak area showing a dominant peptide peak
Mass spectrometry (MS) confirms correct mass for peptide sequence
Bioactivity demonstrated via cell migration or proliferation assays in research settings
Analytical Purity:
HPLC showed single major peak with ≥98 % area
Identity confirmed via MS showing matching molecular mass
Endotoxin Levels:
Some research‑grade batches have low endotoxin (<0.1 EU/µg), if tested
Batch‑Specific COA:
Purity percentage by HPLC
MS identity and retention time data
Peptide content per vial
Storage guidance
Lyophilized Powder (Unopened)
Short‑Term: Store at 2‑8 °C (refrigerator)
Long‑Term: Store at ‑20 °C to ‑80 °C (preferred for maximum stability)
Store in a dry, dark, airtight container
Include desiccant to reduce moisture uptake
Reconstituted (After Adding Solvent)
Reconstitute with sterile water or appropriate buffer immediately before use
Short‑Term Use: 2‑8 °C for up to 7 days
Mid‑Term: Freeze single‑use aliquots at ‑20 °C/‑80 °C (avoid repeated freeze‑thaw cycles)
Avoid repeated thawing; cycles degrade peptide integrity
Stability Considerations
Peptides may degrade upon moisture exposure, light, or oxidation
Clear solutions with no particulates indicate good condition
Lyophilized Powder (Unopened)
Short‑Term: Store at 2‑8 °C (refrigerator)
Long‑Term: Store at ‑20 °C to ‑80 °C (preferred for maximum stability)
Store in a dry, dark, airtight container
Include desiccant to reduce moisture uptake
Reconstituted (After Adding Solvent)
Reconstitute with sterile water or appropriate buffer immediately before use
Short‑Term Use: 2‑8 °C for up to 7 days
Mid‑Term: Freeze single‑use aliquots at ‑20 °C/‑80 °C (avoid repeated freeze‑thaw cycles)
Avoid repeated thawing; cycles degrade peptide integrity
Stability Considerations
Peptides may degrade upon moisture exposure, light, or oxidation
Clear solutions with no particulates indicate good condition